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Cation determination is important for quality control of beverage products. To determine a large group simultaneously, a capillary electrophoresis
procedure is developed with indirect UV at 214 nm in a three-complex buffer system (10mM N,N-dimethylbenzylamine (DBA), 8mM lactic acid
and 2mM 18-crown-6) with good mobility matching with desired cations. Under optimized conditions with pH adjusted to 4.65, a baseline
separation is achieved for 14 cations (Rb+, NH4
+, K+, Ca2+, Na+, Mg2+, Mn2+, Co2+, Fe2+, Cd2+, Cr3+, Ni2+, Zn2+ and Cu2+) within 7 min using
an uncoated silica column. To cover ng/l to
g/l range, both hydrostatic and electrokinetic sampling are studied, showing working ranges within
(0.05–50)/(0.005–2)
g/l and detection limits (13–78)/(1.4–10) ng/l, respectively with satisfactory repeatability (RSD 0.31–0.47% for migration
time, and 3.0–4.0% for peak height measurement). Agreeable results with established inductively coupled plasma-atomic emission spectrometry
method have been obtained for orange juice and tea samples.




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Create Date : 17 กรกฎาคม 2553		2 comments
Last Update : 17 กรกฎาคม 2553 14:44:17 น.
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