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Restriction Enzyme Digestion
Restriction Enzyme Digestion
(check for colonies with insert)
Enzyme 0.2 µl
10x Buffer 2 µl
100x BSA Depending on enzyme
Miniprep DNA 1 2 µl
DW Volume up to 20 µl
Incubate the reaction at 37 ◦C for 1 3 hour
| Create Date : 28 มกราคม 2551 |
| Last Update : 28 มกราคม 2551 16:01:36 น. |
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If there is a stage at which an individual life becomes truly adult, it must be when one grasps the irony in its unfolding and accepts responsibility for a life lived in the midst of such paradox. One must live in the middle of contradiction, because if all contradiction were eliminated at once life would collapse. There are simply no answers to some of the great pressing questions. You continue to live them out, making your life a worthy expression of leaning into the light.
-Barry Lopez Arctic Dreams
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