|
|
Repairing 3 or 5 Overhanging End to Generate Blunt End
Repairing 3 or 5 Overhanging End to Generate Blunt End
1. Digest 0.1 4 µg DNA with appropriate restriction enzyme
2. Purifythe required DNA band from gel after digestion if necessary
3. Add dNTP and EcoPol Buffer
4. Add 1-5 U of Klenow enzyme and incubate at RT for 30 minutes
DNA x µl
10x EcoPol Buffer 5 µl
0.5 mM dNTP 1 µl
Klenow 1 µl
H2O 3 µl
Total Volume = 50 µl
5. Stop reaction either by heating at 75◦C for 20 minutes or by adding 1 µl of 0.5 M EDTA or purify through column (preferred)
| Create Date : 28 มกราคม 2551 |
| Last Update : 28 มกราคม 2551 15:56:58 น. |
|
0 comments
|
| Counter : 295 Pageviews. |
 |
|
|
|
|
|
|
|
Location :
[Profile ทั้งหมด]
|
 ฝากข้อความหลังไมค์
 Rss Feed
 ผู้ติดตามบล็อก : 1 คน [?]
|
If there is a stage at which an individual life becomes truly adult, it must be when one grasps the irony in its unfolding and accepts responsibility for a life lived in the midst of such paradox. One must live in the middle of contradiction, because if all contradiction were eliminated at once life would collapse. There are simply no answers to some of the great pressing questions. You continue to live them out, making your life a worthy expression of leaning into the light.
-Barry Lopez Arctic Dreams
|
|
|
|
|
|
|
|
|
