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Recipe ETC.
LB Agar Plate
Bactotryptone/Casine 10 g
Yeast Extract 5 g
NaCl 10 g
Bacteria Agar 15 g
Distilled Water to volume 1000 ml
Autoclave --> Let cool to ~50 ◦C
Ampicillin (50mg/ml) 1 g
LB Broth
Bactotryptone/Casine 10 g
Yeast Extract 5 g
NaCl 10 g
Distilled Water to volume 1000 ml
Autoclave --> Let cool to ~50 ◦C
Ampicillin (50mg/ml) 1 g
S.O.C Medium
Bactotryptone/Casine 2 g
Yeast Extract 0.5 g
1M NaCl 1 g
1M KCl 0.25 g
Distilled Water to volume 100 ml
Autoclave --> Let cool to RT --> Then add
20mM Mg2+
20mM Glucose
Filter medium through 0.2 µm filter, pH7.0
X-Gal Plate
X-Gal (50mg/ml) 20 µl
0.1 M IPTG 50 µl
H2O 30 µl
Total Volume = 100 µl --> spread on LB/Amp plate
OR
Spread 40 µl of 50mg/ml X-Gal straight onto LB/Amp plate.
0.5 M EDTA (pH 8.0)
Na2EDTA.2H2O 186.1 g
DW Volume up to 1 L
Bring pH to 8 with NaOH pellets (about 20 g); the EDTA will not dissolve until the pH is about right. Bring volume to 1 liter with dH2O and autoclave.
TAE Buffer
50X Stock Solution
Tris base 242 g
Glacial acetic acid 57.1 ml
0.5 M EDTA (pH 8.0) 100 ml
DW Volume up to 1
| Create Date : 28 มกราคม 2551 |
| Last Update : 28 มกราคม 2551 15:56:39 น. |
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If there is a stage at which an individual life becomes truly adult, it must be when one grasps the irony in its unfolding and accepts responsibility for a life lived in the midst of such paradox. One must live in the middle of contradiction, because if all contradiction were eliminated at once life would collapse. There are simply no answers to some of the great pressing questions. You continue to live them out, making your life a worthy expression of leaning into the light.
-Barry Lopez Arctic Dreams
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